Erhardt Publications
Goldlust K, Ducret A, M Halte, Dedieu-Berne A, Erhardt M and Lesterlin C. The F pilus serves as a conduit for the DNA during conjugation between physically distant bacteria.
PNAS (2023) 120(47):e2310842120 doi.org/10.1073/pnas.2310842120
Saleh DO, Horstmann JA, Giralt-Zúñiga M, Weber W, Kaganovitch E, Durairaj AC, Klotzsch E, Strowig T and Erhardt M. SPI-1 virulence gene expression modulates motility of Salmonella Typhimurium in a proton motive force- and adhesins-dependent manner.
PLoS Pathogens. (2023), vol. 19 (6), e1011451 doi.org/10.1371/journal.ppat.1011451
Naseri G, Raasch H, Charpentier E, Erhardt M. A versatile regulatory toolkit of arabinose-inducible artificial transcription factors for Enterobacteriaceae.
Communications Biology. (2023), vol. 6 (1), 1005 doi.org/10.1038/s42003-023-05363-3
Hu H, Popp PF, Santiveri M, Roa-Eguiara A, Yan Y, Martin FJO, Liu Z, Wadhwa N, Wang Y, Erhardt M and Taylor NMI. Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit.
Nature Communications. (2023), vol. 14 (1), 4411. doi.org/10.1038/s41467-023-39899-z
Buttress JA, Halte M, te Winkel JD, Erhardt M, Popp PF and Strahl H. A guide for membrane potential measurements in Gram-negative bacteria using voltage-sensitive dyes.
Microbiology (2022), vol. 168 (9), 001227 doi.org/10.1099/mic.0.001227
Halte M, Wörmann ME, Bogisch M, Erhardt M and Tschowri N. BldD‐based bimolecular fluorescence complementation for in vivo detection of the second messenger cyclic di‐GMP.
Molecular Microbiology (2022), vol. 117 (3), 705–713 doi.org/10.1111/mmi.14876
Guse A, Halte M, Hüsing S and Erhardt M. Hook‐basal‐body assembly state dictates substrate specificity of the flagellar type‐III secretion system.
Molecular Microbiology (2021), vol. 116 (4), 1189–1200 doi:10.1111/mmi.14805
Hüsing S, Halte M, van Look U, Guse A, Galvez EJC, Charpentier E, Blair DF, Erhardt M and Renault TT. Control of membrane barrier during bacterial type-III protein secretion.
Nature Communications (2021) vol. 12, 3999 doi:10.1038/s41467-021-24226-1
Santiveri M, Roa-Eguiara A, Kühne C, Wadhwa N, Berg HC, Erhardt M and Taylor NMI. Structure and function of stator units of the bacterial flagellar motor.
Cell (2020) vol. 183, 244-257.e16 doi:10.1016/j.cell.2020.08.016
Cazzola H, Lemaire L, Acket S, Prost E, Duma L, Erhard M, Čechová P, Trouillas P, Mohareb F, Rossi C and Rossez Y. The impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagic Escherichia coli.
mSphere (2020) vol 5, e00702-20 doi:10.1128/mSphere.00702-20
Horstmann JA, Lunelli M, Cazzola H, Heidemann J, Kühne C, Steffen P, Szefs S, Rossi C, Lokareddy RK, Wang C, Lemaire L, Hughes KT, Uetrecht C, Schlüter H, Grassl GA, Stradal TEB, Rossez Y, Kolbe M and Erhardt M. Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion.
Nature Communications (2020) vol. 11, 2013 doi:10.1038/s41467-020-15738-3
Felgner S, Spöring I, Pawar V, Kocijancic D, Preusse M, Falk C, Rhode M, Häussler S, Weiss S and Erhardt M. The immunogenic potential of bacterial flagella for Salmonella-mediated tumor therapy.
International Journal of Cancer (2020) 10.1002/ijc.32807 doi:10.1002/ijc.32807
Nakamura S, Hanaizumi Y, Morimoto YV, Inoue Y, Erhardt M, Minamino T and Namba K. Direct observation of speed fluctuations of flagellar motor rotation at extremely low load close to zero.
Molecular Microbiology (2019) vol. 113, 755-765 doi:10.1111/mmi.14440
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C and Kolbe M. Molecular organization of soluble type III secretion system sorting platform complexes.
Journal of Molecular Biology (2019) vol. 431, 3787-803 doi:10.1016/j.jmb.2019.07.004
Spöring I, Martinez VA, Hotz C, Schwarz-Linek J, Grady KL, Nava-Sedeno JM, Vissers T, Singer HM, Rhode M, Bourquin C, HatzikirouH, Poon WC, Dufour Y and Erhardt M. Hook length of the bacterial flagellum is optimized for maximal stability of the flagellar bundle.
PLOS Biology (2018) vol. 16 (9) e2006989 doi:10.1371/journal.pbio.2006989
Dongre M, Singh B, Aung KM, Larsson P, Miftakhova R, Persson K, Askarian F, Johannessen M, von Hofsten J, Persson JL, Erhardt M, Tuck S, Uhlin BE and Wai SN. Flagella-mediated secretion of a novel Vibrio cholerae cytotoxin affecting both vertebrate and invertebrate hosts.
Communications Biology (2018) vol. 1 59 doi:10.1128/mBio.00736-17
Spöring I, Felgner S, Preusse M, Eckweiler D, Rhode M, Häussler S, Weiss S and Erhardt M. Regulation of Flagellum Biosynthesis in Response to Cell Envelope Stress in Salmonella enterica Serovar Typhimurium.
mBio (2018) vol. 9 (3) e00736-17 doi:10.1128/mBio.00736-17
Ward E, Renault TT, Kim EA, Erhardt M, Hughes KT and Blair DF. Type-III Secretion Pore Formed by Flagellar Protein FliP.
Molecular Microbiology (2018) vol. 107 (1) 94?103 doi:10.1111/mmi.13870
Felgner S, Kocijancic D, Frahm M, Heise U, Zimmermann K, Erhardt M and Weiss S. Engineered Salmonella enterica serovar Typhimurium overcome limitations of anti-bacterial immunity in bacteria-mediated tumor therapy.
OncoImmunology (2018) vol. 7 (2) e1382791 doi:10.1080/2162402X.2017.1382791
Fabiani FD, Renault TT, Peters B, Dietsche T, Galvez EJC, Guse A, Freier K, Charpentier E, Strowig T, Franz-Wachtel M, Macek B, Wagner S, Hensel M and Erhardt M. A flagellum-specific chaperone facilitates assembly of the core type-III export apparatus of the bacterial flagellum.
PLOS Biology (2017) vol. 15 (8) e2002267 doi:10.1371/journal.pbio.2002267
Thiemann S, Smit N, Schauer T, Roy U, Gàlvez EJC, Helmecke J, Lesker TR, Basic M, Bleich A, Goodmann A, Kalinke U, Flavell RA, Erhardt M and Strowig T. Enhancement of IFN-gamma production in CD4+ T cells by distinct commensals ameliorates Salmonella induced disease.
Cell Host & Microbe (2017) 10.1016/j.chom.2017.05.005 doi:10.1016/j.chom.2017.05.005
Kocijancic, D Felgner S, Schauer T, Frahm M, Heise U, Zimmermann K, Erhardt M and Weiss S. Local application of bacteria improves safety of Salmonella-mediated tumor therapy and retains advantages of systemic infection.
Oncotarget (2017) 10.18632/oncotarget.18392 doi:/10.18632/oncotarget.18392
Paradis G, Chevance FFV, Liou W, Renault TT, Hughes KT, Rainville S and Erhardt M. Variability in bacterial flagella re-growth patterns after breakage.
Scientific Reports (2017) 10.1038/s41598-017-01302-5 doi:10.1038/s41598-017-01302-5
Horstmann J, Zschieschang E, Truschel T, de Diego J, Lunelli M, Rohde M, May T, Strowig T, Stradal T, Kolbe M and Erhardt M. Flagellin phase-dependent swimming on epithelial cell surfaces contributes to productive Salmonella gut colonization.
Cellular Microbiology (2017) 10.1111/cmi.12739 doi:10.1111/cmi.12739
Renault TT, Abraham AO, Bergmiller T, Paradis G, Rainville S, Charpentier E, Guet CC, Tu Y, Namba K, Keener JP, Minamino T and Erhardt M. Bacterial flagella grow through an injection-diffusion mechanism.
eLife (2017) 10.7554/eLife.23136 doi:10.7554/eLife.23136
Erhardt M, Wheatley P, Kim EA, Hirano T, Zhang Y, Sarkar MK, Hughes KT and Blair DF. Mechanism of type-III protein secretion: Regulation of FlhA conformation by a functionally critical charged-residue cluster.
Molecular Microbiology (2017) 10.1111/mmi.13623 doi:10.1111/mmi.13623
Felgner S, Frahm M, Kocijancic D, Rohde M, Eckweiler D, Bielecka A, Bueno E, Cava F, Abraham WR, Curtiss R 3rd, Häussler S, Erhardt M and Weiss S. AroA deficient Salmonella Typhimurium is more than a metabolically attenuated mutant.
mBio (2016) vol. 7 (5) e01220-16 doi:10.1128/mBio.01220-16
Barlag B, Beutel O, Janning D, Czarniak F, Richter CP, Kurre R, Fabiani F, Erhardt M, Piehler J and Hensel M. Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labeling enzymes.
Scientific Reports (2016) vol. 6 p.31601 doi:10.1038/srep31601
Kühne C, Singer HM, Grabisch, Codutti L, Carlomagno T, Scrima A and Erhardt M. RflM mediates target specificity of the RcsCDB phosphorelay system for transcriptional repression of flagellar synthesis in Salmonella enterica.
Molecular Microbiology (2016) vol. 101 (5) 841-55 doi:10.1111/mmi.13427
Felgner S, Kocijancic D, Frahm M, Curtiss R 3rd, Erhardt M and Weiss S. Optimizing Salmonella enterica Serovar Typhimurium for Bacteria-mediated Tumor Therapy.
Gut Microbes (2016) vol. 7 (2) 177-77 doi:10.1080/19490976.2016.1155021
Deditius JA, Felgner S, Spöring I, Kühne C, Frahm M, Rohde M, Weiss S and Erhardt M. Characterization of novel factors involved in swimming and swarming motility in Salmonella enterica serovar Typhimurium.
PLOS ONE (2015) vol. 10 (8) e0135351 doi:10.1371/journal.pone.0135351
Frahm M, Felgner S, Kocijancic D, Rohde M, Hensel M, Curtiss R 3rd, Erhardt M and Weiss S. Efficiency of conditionally attenuated Salmonella Typhimurium in bacteria mediated tumour therapy.
mBio (2015) vol. 6 (2) e00254-15 doi:/10.1128/mBio.00254-15
Hendrix RW, Ko CC, Jacobs-Sera D, Hatfull GF, Kropinski AM, Denyes JM, Erhardt M, Hughes KT and Casjens SR. The genome sequence of Salmonella phage Chi.
Genome Announcements (2015) vol. 3 (1) e01229-14 doi:10.1128/genomeA.01229-14
Erhardt M, Mertens ME, Fabiani FD and Hughes KT. ATPase-independent type-III protein secretion in Salmonella enterica.
PLOS Genetics (2014) vol. 10 (11) e1004800 doi:10.1371/journal.pgen.1004800
Singer HM, Erhardt M and Hughes KT. Comparative analysis of the secretion capability of early and late flagellar type III secretion substrates.
Molecular Microbiology (2014) vol. 93, 505-20 doi:10.1111/mmi.12675
Singer HM, Kühne C, Deditius JA, Hughes KT and Erhardt M. The Salmonella Spi1 virulence regulatory protein HilD directly activates transcription of the flagellar master operon flhDC.
Journal of Bacteriology (2014) vol. 196, 1448-57 doi:10.1128/JB.01438-13
Singer HM, Erhardt M and Hughes KT. RflM functions as a transcriptional repressor in the autogenous control of the Salmonella flagellar master operon flhDC.
Journal of Bacteriology (2013) vol. 195, 4274-82. doi:10.1128/JB.00728-13
Singer HM, Erhardt M, Steiner AM, Zhang MM, Yoshikami D, Bulaj G, Olivera BM and Hughes KT. Selective purification of recombinant neuroactive peptides using the flagellar type III secretion system.
mBio (2012) vol. 29 (3) e00115-12 doi:10.1128/mBio.00115-12
Takaya A, Erhardt M, Karata K, Winterberg K, Yamamoto T and Hughes KT. YdiV: a dual function protein that targets FlhDC for ClpXP-dependent degradation by promoting release of DNA-bound FlhDC complex.
Molecular Microbiology (2012) vol. 83 (6) 1268-84 doi:10.1111/j.1365-2958.2012.08007.x
Erhardt M, Singer HM, Wee DH, Keener JP and Hughes KT. An infrequent molecular ruler controls flagellar hook length in Salmonella enterica.
The EMBO Journal (2011) vol. 30 2948-61 doi:10.1038/emboj.2011.185
Erhardt M, Hirano T, Su Y, Paul K, Wee DH, Mizuno S, Aizawa SI and Hughes KT. The role of the FliK molecular ruler in hook-length control in Salmonella enterica.
Molecular Microbiology (2010) vol. 75 (5) 1272-84 doi:10.1111/j.1365-2958.2010.07050.x
Erhardt M and Hughes KT. C-ring requirement in flagellar type III secretion is bypassed by FlhDC upregulation.
Molecular Microbiology (2010) vol. 75 (2) 376-93 doi:10.1111/j.1365-2958.2009.06973.x
Koplin A, Preissler S, Ilina Y, Koch M, Scior A, Erhardt M and Deuerling E. A dual function for chaperones SSB-RAC and the NAC nascent polypeptide-associated complex on ribosomes.
Journal of Cell Biology (2010) vol. 189 (1) 57-68 doi:10.1083/jcb.200910074
Erhardt M, Wegrzyn RD and Deuerling E. Extra N-Terminal Residues Have a Profound Effect on the Aggregation Properties of the Potential Yeast Prion Protein Mca1.
PLOS ONE (2010) vol. 5 (3) e9929 EP doi:10.1371/journal.pone.0009929
Paul K, Erhardt M, Hirano T, Blair DF and Hughes KT. Energy source of flagellar type III secretion.
Nature (2008) vol. 451 (7177) 489-92 doi:10.1038/nature06497
Chevance FFV, Erhardt M, Lengsfeld C, Lee SJ and Boos W. Mlc of Thermus thermophilus: a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System.
Journal of Bacteriology (2006) vol. 188 (18) 6561-71 doi:10.1128/JB.00715-06
Reviews
Naseri G. A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast.
Nature Communications (2023) vol. 14, 1916 doi:10.1038/s41467-023-37627-1
Hu H, Santiveri M, Wadhwa N, Berg HC, Erhardt M and Taylor NMI. Structural basis of torque generation in the bi-directional bacterial flagellar motor.
Trends in Biochemal Sciences (2022) vol. 47 (2), 160-172 doi:10.1016/j.tibs.2021.06.005
Popp PF and Erhardt M. With or without you: crosstalk between cell division and flagellum assembly.
Developmental Cell (2021) vol. 56 (5), 573-574 doi:10.1016/j.devcel.2021.02.013
Halte M and Erhardt M. Protein Export via the Type III Secretion System of the Bacterial Flagellum.
Biomolecules (2021) vol. 11 (2), 186 doi:10.3390/biom11020186
Renault TT, Guse A and Erhardt M. Export Mechanisms and Energy Transduction in Type-III Secretion Machines.
Current Topics in Microbiology and Immunology (2019) doi:10.1007/82_2019_166
Miranda-CasoLuengo AA, Kary SC, Erhardt M and Kröger C. Small RNA, big effect: control of flagellin production.
Trends in Microbiology (2017) S0966-842X(17)30230-5 doi:10.1016/j.tim.2017.10.003
Felgner S, Pawar V, Kocijancic D, Erhardt M and Weiss S. Tumour-targeting bacteria-based cancer therapies for increased specificity and improved outcome.
Microbial Biotechnology (2017) vol. 10 1074-1078 doi:10.1111/1751-7915.12787
Erhardt M. Strategies to block bacterial pathogenesis by interference with motility and chemotaxis.
Current Topics in Microbiology and Immunology (2016) vol. 398 185-205 doi:10.1007/82_2016_493
Erhardt M and Dersch P. Regulatory principles governing Salmonella and Yersinia virulence.
Frontiers in Microbiology Review (2015) vol. 6 no. 00949 doi:10.3389/fmicb.2015.00949
Erhardt M, Namba K and Hughes KT. Bacterial Nanomachines: The Flagellum and Type III Injectisome.
Cold Spring Harbor Perspectives in Biology (2010) vol. 2 (11) a000299 doi:10.1101/cshperspect.a000299
Book Chapters
Kühne C, Guse A, Hüsing S and Erhardt M. Bacterial Flagella.
eLS. John Wiley & Sons Ltd, Chichester (2020) 10.1002/9780470015902.a0000301.pub3
doi:10.1002/9780470015902.a0000301.pub3
Erhardt M. Energy requirements for protein secretion via the flagellar type-III secretion system.
Methods in Molecular Biology - Bacterial Protein Secretion Systems, Springer New York (2017) 449-457
doi:10.1007/978-1-4939-7033-9_30
Erhardt M. Fluorescent microscopy techniques to study hook length control and flagella formation.
Methods in Molecular Biology - The Bacterial Flagellum: Methods and Protocols, Springer New York (2017) 37-46
doi:10.1007/978-1-4939-6927-2_3
Hughes KT and Erhardt M. Bacterial Flagella.
eLS. John Wiley & Sons Ltd, Chichester (2011) 10.1002/9780470015902.a0000301.pub2
doi:10.1002/9780470015902.a0000301.pub2